Hotspot

rpoC2

LSC · 15628-19869 · 4242 bp

Back to Tinospora

Pi 0.456

MarkerSeek score 75.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4242
variable_sites: 1567
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACGGTCGAAGCCATTACTTC	CCCATTTCGAGGACTCACAG	50.0	55.0	58.0	58.0	803 bp	0.5	59.8
rpoC2_p2	ACGGTCGAAGCCATTACTTC	ATTGGTTGTGCTCGAAAGGT	50.0	45.0	58.0	58.0	952 bp	0.5	59.8
rpoC2_p3	ACGGTCGAAGCCATTACTTC	CCCGGTTCCCCAATAGATTG	50.0	55.0	58.0	58.0	1093 bp	0.5	59.8
rpoC2_p4	GGGTCGATCTGGTCTTTCAC	CCCATTTCGAGGACTCACAG	55.0	55.0	58.0	58.0	689 bp	0.5	59.8
rpoC2_p5	GGGTCGATCTGGTCTTTCAC	ATTGGTTGTGCTCGAAAGGT	55.0	45.0	58.0	58.0	838 bp	0.5	59.8