Hotspot

psbM

LSC · 28663-28779 · 117 bp

Back to Tinospora

Pi 0.453

MarkerSeek score 72.60

Primer available yes

Label psbM

Feature Metadata

label_name: psbM
feature_type: gene
region: LSC
length_bp: 117
variable_sites: 53
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM_p1	CGAGAGCAACTAGAGTCGTC	CGTGCTGCTCTCACTAAAGT	55.0	50.0	57.6	57.9	1245-2469 bp	1.0	79.7
psbM_p2	TGATGATTCTAGCCCGCTCA	CGTGCTGCTCTCACTAAAGT	50.0	50.0	58.6	57.9	867-1642 bp	1.0	79.1
psbM_p3	CCCTCTCTACTGTTCAGGGA	CGTGCTGCTCTCACTAAAGT	55.0	50.0	57.5	57.9	1309-2533 bp	1.0	78.9
psbM_p4	TCCCTCTCTACTGTTCAGGG	CGTGCTGCTCTCACTAAAGT	55.0	50.0	57.5	57.9	1310-2534 bp	1.0	78.9
psbM_p5	CGAGAGCAACTAGAGTCGTC	ATAGACAATAGCCGTGCTGC	55.0	50.0	57.6	58.1	1257 bp	0.5	55.4