Hotspot

rps15-ycf1

SSC · 120729-121179 · 451 bp

Back to Tinospora

Pi 0.0312

MarkerSeek score 74.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 451
variable_sites: 14
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AATGTGCAGTAAGTCTCCGT	GGCTACATATGTATCCGCGA	45.0	50.0	56.9	57.1	604-608 bp	1.0	65.6
rps15-ycf1_p2	AATGTGCAGTAAGTCTCCGT	AGGCTACATATGTATCCGCG	45.0	50.0	56.9	57.1	605-609 bp	1.0	65.5
rps15-ycf1_p3	AAATGTGCAGTAAGTCTCCGT	GGCTACATATGTATCCGCGA	42.9	50.0	57.6	57.1	605-609 bp	1.0	63.4
rps15-ycf1_p4	AAATGTGCAGTAAGTCTCCGT	AGGCTACATATGTATCCGCG	42.9	50.0	57.6	57.1	606-610 bp	1.0	63.4
rps15-ycf1_p5	AATGTGCAGTAAGTCTCCGT	AAGGCTACATATGTATCCGCG	45.0	47.6	56.9	57.8	606-610 bp	1.0	63.2