Hotspot

atpB-rbcL

LSC · 53501-54309 · 809 bp

Back to Tinospora

Pi 0.0115
MarkerSeek score 68.60
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
809
variable_sites
9
pi
0.0115
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CATCCAGTACCGGACCAATG GGTGACATAAGTCCCTCCCT 55.0 55.0 58.0 58.1 912-935 bp 1.0 81.8
atpB-rbcL_p2 TCTTCAAGTGCGGAAACCTC GGTGACATAAGTCCCTCCCT 50.0 55.0 58.1 58.1 865-888 bp 1.0 81.4
atpB-rbcL_p3 ATCCAGTACCGGACCAATGA GGTGACATAAGTCCCTCCCT 50.0 55.0 58.1 58.1 911-934 bp 1.0 81.2
atpB-rbcL_p4 TCCAGTACCGGACCAATGAT GGTGACATAAGTCCCTCCCT 50.0 55.0 58.1 58.1 910-933 bp 1.0 81.2
atpB-rbcL_p5 ACTACATCCAGTACCGGACC GGTGACATAAGTCCCTCCCT 55.0 55.0 58.2 58.1 916-939 bp 1.0 80.3