Hotspot

psbI-trnS-GCT

LSC · 7572-7711 · 140 bp

Back to Tinospora

Pi 0.0357

MarkerSeek score 69.00

Primer available yes

Label psbI-trnS-GCT

Feature Metadata

label_name: psbI-trnS-GCT
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 5
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCT_p1	CCCCCATGCTTACTCTCAAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.2	58.0	324 bp	0.5	53.2
psbI-trnS-GCT_p2	CCCCCATGCTTACTCTCAAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.2	58.1	348 bp	0.5	52.7
psbI-trnS-GCT_p3	CCCCCATGCTTACTCTCAAA	ATGGCTGAGTGGACTAAAGC	50.0	50.0	57.2	57.6	338 bp	0.5	50.3
psbI-trnS-GCT_p4	CCCCCATGCTTACTCTCAAA	GAGAGATGGCTGAGTGGACT	50.0	55.0	57.2	58.5	343 bp	0.5	49.4
psbI-trnS-GCT_p5	CCCCATGCTTACTCTCAAACT	TAAAGCGGCGGATTGCTAAT	47.6	45.0	57.6	58.0	323 bp	0.5	48.8