Hotspot

trnH-GTG-psbA

LSC · 133-304 · 172 bp

Back to Tinospora

Pi 0.064

MarkerSeek score 83.00

Primer available yes

Label trnH-GTG-psbA

Feature Metadata

label_name: trnH-GTG-psbA
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 11
pi: 0.064
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	CTGCCTTAATCCACTTGGCT	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	300-658 bp	1.0	79.4
trnH-GTG-psbA_p2	CTGCCTTAATCCACTTGGCT	AAGCCCCATCTACAAATGGC	50.0	50.0	57.9	58.2	223-581 bp	1.0	79.4
trnH-GTG-psbA_p3	CTGCCTTAATCCACTTGGCT	ATCAACCGTGCTAACCTTGG	50.0	50.0	57.9	58.2	302-660 bp	1.0	79.3
trnH-GTG-psbA_p4	ACTGCCTTAATCCACTTGGC	CAACCGTGCTAACCTTGGTA	50.0	50.0	58.2	57.8	301-659 bp	1.0	79.2
trnH-GTG-psbA_p5	ACTGCCTTAATCCACTTGGC	ATCAACCGTGCTAACCTTGG	50.0	50.0	58.2	58.2	303-661 bp	1.0	79.1