Hotspot

rps8-rpl14

LSC · 125536-125707 · 172 bp

Back to Tillandsia

Pi 0.027

MarkerSeek score 65.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 45
pi: 0.027
unique_haplotype_count: 108
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGATCGGCAATAGTGTCCCT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.2	58.0	260-295 bp	0.982143	89.5
rps8-rpl14_p2	AGATCGGCAATAGTGTCCCT	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	58.2	58.1	258-293 bp	0.982143	88.7
rps8-rpl14_p3	AGATCGGCAATAGTGTCCCT	GGAACTCGAGTTTTTGGTGC	50.0	50.0	58.2	57.6	261-296 bp	0.994048	86.9
rps8-rpl14_p4	AGATCGGCAATAGTGTCCCT	AACTCGAGTTTTTGGTGCGA	50.0	45.0	58.2	58.3	259-294 bp	0.982143	86.9
rps8-rpl14_p5	AGATCGGCAATAGTGTCCCT	CTCGAGTTTTTGGTGCGATC	50.0	50.0	58.2	57.5	257-292 bp	0.97619	85.3