Hotspot

psaJ-rpl33

LSC · 111966-112489 · 524 bp

Back to Tillandsia

Pi 0.0109

MarkerSeek score 68.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 88
pi: 0.0109
unique_haplotype_count: 80
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	CCCGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	545-703 bp	1.0	91.1
psaJ-rpl33_p2	TATTCCCAGATGCCCTGTCA	CCCGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	506-664 bp	1.0	90.7
psaJ-rpl33_p3	CGTGGCACCTGTGTTAACTA	CCCGCGATTCCTTATTGACA	50.0	50.0	57.8	58.1	574-732 bp	1.0	90.3
psaJ-rpl33_p4	TGGGTCTTTAGCGGGTCTAT	CCCGCGATTCCTTATTGACA	50.0	50.0	57.8	58.1	544-702 bp	1.0	90.2
psaJ-rpl33_p5	ATGGTTTGGGTCTTTAGCGG	CCCGCGATTCCTTATTGACA	50.0	50.0	58.2	58.1	550-708 bp	0.994048	90.0