Hotspot

petA-psbJ

LSC · 107270-108403 · 1134 bp

Back to Tillandsia

Pi 0.0122

MarkerSeek score 74.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 165
pi: 0.0122
unique_haplotype_count: 113
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.9	58.0	641-1312 bp	1.0	91.2
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.9	58.0	641-1310 bp	1.0	91.2
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	50.0	55.0	57.9	57.6	642-1311 bp	1.0	88.3
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.7	58.0	648-1319 bp	1.0	86.2
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.7	58.0	648-1317 bp	1.0	86.2