Hotspot

trnS-GCU-trnG-UCC

LSC · 50244-51438 · 1195 bp

Back to Tillandsia

Pi 0.0154

MarkerSeek score 65.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1195
variable_sites: 263
pi: 0.0154
unique_haplotype_count: 133
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AACCGATGGACCCCTTAACT	45.0	50.0	58.0	58.3	883-1365 bp	1.0	89.0
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AAACCGATGGACCCCTTAAC	45.0	50.0	58.0	57.5	884-1366 bp	1.0	87.7
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	ACCGATGGACCCCTTAACTA	45.0	50.0	58.0	57.4	882-1364 bp	1.0	87.2
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	AACCGATGGACCCCTTAACT	50.0	50.0	57.6	58.3	869-1351 bp	1.0	86.0
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	AACCGATGGACCCCTTAACT	55.0	50.0	58.5	58.3	864-1346 bp	1.0	85.2