Hotspot

ccsA-ndhD

SSC · 2835-3099 · 265 bp

Back to Tillandsia

Pi 0.0386
MarkerSeek score 63.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
265
variable_sites
55
pi
0.0386
unique_haplotype_count
61
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGGTCTACATAGTTATGGTTCA CAGTTGACAAGGTACACGCT 36.4 50.0 53.7 58.1 337-365 bp 0.994048 51.8
ccsA-ndhD_p2 AGGTCTACATAGTTATGGTTCA TCAGTTGACAAGGTACACGC 36.4 50.0 53.7 58.1 338-366 bp 0.994048 51.8
ccsA-ndhD_p3 AGGTCTACATAGTTATGGTTCATTCA CAGTTGACAAGGTACACGCT 34.6 50.0 57.7 58.1 337-365 bp 0.97619 51.0
ccsA-ndhD_p4 AGGTCTACATAGTTATGGTTCATTCA TCAGTTGACAAGGTACACGC 34.6 50.0 57.7 58.1 338-366 bp 0.97619 51.0
ccsA-ndhD_p5 GGTCTACATAGTTATGGTTCATTCAC CAGTTGACAAGGTACACGCT 38.5 50.0 57.6 58.1 336-364 bp 0.97619 51.0