Hotspot

rps16

LSC · 5589-6714 · 1126 bp

Pi 0.00271

MarkerSeek score 53.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1126
variable_sites: 7
pi: 0.00271
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAGTCATCCACACCCTCTT	TGTATCGACTGAACCAATGACT	50.0	40.9	58.0	57.2	1226-1229 bp	1.0	57.9
rps16_p2	ATGAGTCATCCACACCCTCT	TGTATCGACTGAACCAATGACT	50.0	40.9	57.7	57.2	1227-1230 bp	1.0	56.1
rps16_p3	TGAGTCATCCACACCCTCTT	TGTATCGACTGAACCAATGAC	50.0	42.9	58.0	55.7	1226-1229 bp	1.0	54.2
rps16_p4	TGAGTCATCCACACCCTCTT	ATGTATCGACTGAACCAATGACT	50.0	39.1	58.0	57.7	1227-1230 bp	1.0	53.6
rps16_p5	TGAGTCATCCACACCCTCTT	TCGACTGAACCAATGACTATTCA	50.0	39.1	58.0	57.6	1222-1225 bp	1.0	53.1