Hotspot

ccsA-ndhD

SSC · 122818-123111 · 294 bp

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Pi 0.0133
MarkerSeek score 50.00
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
294
variable_sites
12
pi
0.0133
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GTAAATTCCGCACTTGTGGC ATCCGGATTTCGTCCTCTCA 50.0 50.0 58.0 58.2 484-485 bp 1.0 80.2
ccsA-ndhD_p2 GTAAATTCCGCACTTGTGGC TCCGGATTTCGTCCTCTCAT 50.0 50.0 58.0 58.2 483-484 bp 1.0 80.2
ccsA-ndhD_p3 TAAATTCCGCACTTGTGGCT ATCCGGATTTCGTCCTCTCA 45.0 50.0 58.1 58.2 483-484 bp 1.0 79.6
ccsA-ndhD_p4 TAAATTCCGCACTTGTGGCT TCCGGATTTCGTCCTCTCAT 45.0 50.0 58.1 58.2 482-483 bp 1.0 79.6
ccsA-ndhD_p5 GTAAATTCCGCACTTGTGGC CCGGATTTCGTCCTCTCATT 50.0 50.0 58.0 57.4 482-483 bp 1.0 77.1