Hotspot

ndhF-rpl32

SSC · 119055-120356 · 1302 bp

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Pi 0.00446
MarkerSeek score 56.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1302
variable_sites
13
pi
0.00446
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCGTCGGAAAAAGTA TGAAAAAGCTTTCAACGCCG 45.0 45.0 58.0 58.2 1459-1500 bp 1.0 82.3
ndhF-rpl32_p2 TTGGAAGTGGCACAAAAGGT TGAAAAAGCTTTCAACGCCG 45.0 45.0 58.1 58.2 1412-1453 bp 1.0 81.7
ndhF-rpl32_p3 TGTTGCCGTCGGAAAAAGTA GCCGCCCAATATCCTTTCTT 45.0 50.0 58.0 58.3 1443-1484 bp 1.0 81.2
ndhF-rpl32_p4 TTTGTTGCCGTCGGAAAAAG TGAAAAAGCTTTCAACGCCG 45.0 45.0 57.8 58.2 1461-1502 bp 1.0 81.1
ndhF-rpl32_p5 TTGGAAGTGGCACAAAAGGT GCCGCCCAATATCCTTTCTT 45.0 50.0 58.1 58.3 1396-1437 bp 1.0 80.6