Hotspot

trnN-GUU-ndhF

IRb · 116411-116852 · 442 bp

Back to Tilia

Pi 0.00474
MarkerSeek score 55.60
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
442
variable_sites
6
pi
0.00474
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 ACGACCAGTCAGTTAACAGC ATATGTAGGGGGAAGTCGCA 50.0 50.0 57.8 57.9 579-649 bp 1.0 80.9
trnN-GUU-ndhF_p2 ACGACCAGTCAGTTAACAGC TATGTAGGGGGAAGTCGCAT 50.0 50.0 57.8 57.9 578-648 bp 1.0 80.9
trnN-GUU-ndhF_p3 AGGATTCGAACCTACGACCA ATATGTAGGGGGAAGTCGCA 50.0 50.0 58.2 57.9 592-662 bp 1.0 80.8
trnN-GUU-ndhF_p4 AGGATTCGAACCTACGACCA TATGTAGGGGGAAGTCGCAT 50.0 50.0 58.2 57.9 591-661 bp 1.0 80.8
trnN-GUU-ndhF_p5 ACGACCAGTCAGTTAACAGC GGGGAAGTCGCATCTCTTTT 50.0 50.0 57.8 57.9 571-641 bp 1.0 80.8