Hotspot

rps4-trnT-UGU

LSC · 49755-50207 · 453 bp

Back to Tilia

Pi 0.0109
MarkerSeek score 51.80
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
453
variable_sites
10
pi
0.0109
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAAGATCGCTTCCGGCTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 606-622 bp 1.0 81.0
rps4-trnT-UGU_p2 TTTCTAAGATCGCTTCCGGC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 610-626 bp 1.0 80.6
rps4-trnT-UGU_p3 TAAGATCGCTTCCGGCTCTA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 574-590 bp 1.0 80.2
rps4-trnT-UGU_p4 TTTCTAAGATCGCTTCCGGC ATGGTCATCGGTTCGATTCC 50.0 50.0 58.1 57.8 578-594 bp 1.0 79.9
rps4-trnT-UGU_p5 TAAGATCGCTTCCGGCTCTA CCGCTTAGCTCAGAGGTTAG 50.0 55.0 58.0 57.5 614-630 bp 1.0 78.0