Hotspot

ycf3-trnS-GGA

LSC · 47810-48776 · 967 bp

Back to Tilia

Pi 0.00118
MarkerSeek score 54.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
967
variable_sites
3
pi
0.00118
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA TGGCGAATTAGGTGAAGGTG 47.6 50.0 58.0 57.9 1093-1096 bp 1.0 72.2
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA TTAGGTGAAGGTGCGGAAAG 47.6 50.0 58.0 57.8 1086-1089 bp 1.0 71.6
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 1024-1027 bp 1.0 71.6
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA GTGCGGAAAGAGAGGGATTC 47.6 55.0 58.0 58.3 1076-1079 bp 1.0 70.3
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA GAATTAGGTGAAGGTGCGGA 47.6 50.0 58.0 57.6 1089-1092 bp 1.0 69.9