Hotspot

trnR-UCU-atpA

LSC · 10526-10796 · 271 bp

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Pi 0.0102
MarkerSeek score 49.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
271
variable_sites
6
pi
0.0102
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC TCACTGAGGAAGCAGAAACG 47.6 50.0 57.3 57.8 373-380 bp 1.0 66.5
trnR-UCU-atpA_p2 TGGATAGGACAGAGGTCTTCT TCACTGAGGAAGCAGAAACG 47.6 50.0 56.9 57.8 408-415 bp 1.0 63.5
trnR-UCU-atpA_p3 CGTAATGAAAAGCGTCCATTGT TCACTGAGGAAGCAGAAACG 40.9 50.0 57.9 57.8 434-441 bp 1.0 63.2
trnR-UCU-atpA_p4 TCGTAATGAAAAGCGTCCATTG TCACTGAGGAAGCAGAAACG 40.9 50.0 57.7 57.8 435-442 bp 1.0 61.2
trnR-UCU-atpA_p5 ATGAAAAGCGTCCATTGTCT TCACTGAGGAAGCAGAAACG 40.0 50.0 55.6 57.8 430-437 bp 1.0 61.0