Hotspot

ycf1

SSC · 125222-130702 · 5481 bp

Back to Tigridiopalma

Pi 0.00584

MarkerSeek score 48.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 48
pi: 0.00584
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCATGCTTCCGCTATTTCT	AAGACAAACATCGGGTCGTT	50.0	45.0	58.0	57.8	253 bp	1.0	80.0
ycf1_p2	CCCATGCTTCCGCTATTTCT	AGACAAACATCGGGTCGTTT	50.0	45.0	58.0	57.8	252 bp	1.0	80.0
ycf1_p3	TCCCATGCTTCCGCTATTTC	AGACAAACATCGGGTCGTTT	50.0	45.0	58.0	57.8	253 bp	1.0	80.0
ycf1_p4	TCCCATGCTTCCGCTATTTC	AAGACAAACATCGGGTCGTT	50.0	45.0	58.0	57.8	254 bp	1.0	79.9
ycf1_p5	ATGTTATCCCATGCTTCCGC	AGACAAACATCGGGTCGTTT	50.0	45.0	58.4	57.8	259 bp	1.0	77.0