Hotspot

rps16

LSC · 4591-5655 · 1065 bp

Back to Tigridiopalma

Pi 0.0125

MarkerSeek score 65.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1065
variable_sites: 20
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAAAAGAGGGAGGGAGAGGG	TACCAGTTAAAACCCGGGGG	55.0	55.0	58.3	59.3	1171-1172 bp	1.0	69.0
rps16_p2	AAAAAGAGGGAGGGAGAGGG	ATACCAGTTAAAACCCGGGG	55.0	50.0	58.3	57.2	1172-1173 bp	0.666667	57.7
rps16_p3	AGGGAGAGGGGGATCTACTA	ATACCAGTTAAAACCCGGGG	55.0	50.0	57.4	57.2	1162-1163 bp	0.666667	55.9
rps16_p4	AAAAAGAGGGAGGGAGAGGG	ACATACCAGTTAAAACCCGGG	55.0	47.6	58.3	57.9	1174-1175 bp	0.666667	55.6
rps16_p5	CAAAAAGAGGGAGGGAGAGG	ATACCAGTTAAAACCCGGGG	55.0	50.0	57.2	57.2	1173-1174 bp	0.666667	53.9