Hotspot

rps15-ycf1

SSC · 124889-125221 · 333 bp

Back to Tigridiopalma

Pi 0.014

MarkerSeek score 62.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 333
variable_sites: 7
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCCGATTTTCTTCTTCTGA	GGAAGCCGTTGTAGTCTGTT	42.9	50.0	56.7	57.8	437-461 bp	1.0	61.3
rps15-ycf1_p2	TCCCCGATTTTCTTCTTCTGA	ATGGAAGCCGTTGTAGTCTG	42.9	50.0	56.7	57.6	439-463 bp	1.0	59.5
rps15-ycf1_p3	TCCCCGATTTTCTTCTTCTGAA	GGAAGCCGTTGTAGTCTGTT	40.9	50.0	57.4	57.8	437-461 bp	1.0	59.0
rps15-ycf1_p4	ATCCCCGATTTTCTTCTTCTGA	GGAAGCCGTTGTAGTCTGTT	40.9	50.0	57.2	57.8	438-462 bp	1.0	57.4
rps15-ycf1_p5	TCCCCGATTTTCTTCTTCTGAA	ATGGAAGCCGTTGTAGTCTG	40.9	50.0	57.4	57.6	439-463 bp	1.0	57.3