Hotspot

ndhF-rpl32

SSC · 114296-115106 · 811 bp

Back to Tigridiopalma

Pi 0.014

MarkerSeek score 58.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 811
variable_sites: 17
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAATGCTGT	50.0	38.1	58.0	56.9	962-1072 bp	1.0	64.3
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	ATGAAAAAGCCTTCAATGCTGT	50.0	36.4	58.0	57.4	963-1073 bp	1.0	60.2
ndhF-rpl32_p3	TGGGAAGTGGAATGAGAGGT	TCGCTAATGAAAAAGCCTTCA	50.0	38.1	58.0	56.2	969-1079 bp	1.0	58.4
ndhF-rpl32_p4	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAATGCTG	50.0	40.0	58.0	55.1	962-1072 bp	1.0	57.5
ndhF-rpl32_p5	TGGGAAGTGGAATGAGAGGT	GAAAAAGCCTTCAATGCTGT	50.0	40.0	58.0	55.0	961-1071 bp	1.0	57.5