Hotspot

psbE-petL

LSC · 65204-66348 · 1145 bp

Back to Tigridiopalma

Pi 0.00876

MarkerSeek score 64.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 15
pi: 0.00876
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	57.8	57.6	1226-1231 bp	1.0	52.8
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GCTGAGGTTATAGTTAAAGCTGC	45.0	43.5	58.2	57.6	1312-1317 bp	1.0	52.6
psbE-petL_p3	TGCTGACGAATAACCAACCC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.2	57.6	1317-1322 bp	1.0	52.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GCTGAGGTTATAGTTAAAGCTGC	55.0	43.5	58.2	57.6	1221-1226 bp	1.0	52.4
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.4	57.6	1219-1224 bp	1.0	51.2