Hotspot

psbZ-trnG-UCC

LSC · 36881-37191 · 311 bp

Back to Tigridiopalma

Pi 0.0193

MarkerSeek score 44.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 311
variable_sites: 9
pi: 0.0193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	ACCATTCGACTATATCCGC	50.0	47.4	58.1	54.3	412-430 bp	1.0	45.9
psbZ-trnG-UCC_p2	CCCGTTGTTTTTGCTTCTCC	ACCATTCGACTATATCCGC	50.0	47.4	57.9	54.3	433-451 bp	1.0	45.7
psbZ-trnG-UCC_p3	ACGGCTGGTCAAGTAACAAA	ACCATTCGACTATATCCGC	45.0	47.4	57.7	54.3	411-429 bp	1.0	44.9
psbZ-trnG-UCC_p4	TTTTTGCTTCTCCTGACGGC	ACCATTCGACTATATCCGC	50.0	47.4	59.0	54.3	426-444 bp	1.0	44.8
psbZ-trnG-UCC_p5	GTTTTTGCTTCTCCTGACGG	ACCATTCGACTATATCCGC	50.0	47.4	57.3	54.3	427-445 bp	1.0	44.7