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atpH-atpI

LSC · 13745-14695 · 951 bp

Back to Tigridiopalma

Pi 0.00351

MarkerSeek score 65.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 5
pi: 0.00351
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	1023-1057 bp	1.0	81.3
atpH-atpI_p2	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	1035-1069 bp	1.0	81.2
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.1	57.9	991-1025 bp	1.0	81.2
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.1	58.1	1003-1037 bp	1.0	81.1
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.8	57.9	1001-1035 bp	1.0	80.6