Hotspot

rps2

LSC · 17434-18144 · 711 bp

Back to Tieghemella

Pi 0.00141

MarkerSeek score 52.40

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TGAGGTTCACAAATAGTAACCA	GCCAATCCACGGTAGAAGAG	36.4	55.0	55.0	58.1	900-901 bp	1.0	40.2
rps2_p2	TGAGGTTCACAAATAGTAACCAA	GCCAATCCACGGTAGAAGAG	34.8	55.0	55.8	58.1	900-901 bp	1.0	40.2
rps2_p3	TTGAGGTTCACAAATAGTAACCA	GCCAATCCACGGTAGAAGAG	34.8	55.0	55.8	58.1	901-902 bp	1.0	40.2
rps2_p4	GGTTCACAAATAGTAACCAATTCCA	GCCAATCCACGGTAGAAGAG	36.0	55.0	57.8	58.1	897-898 bp	1.0	40.2
rps2_p5	TGAGGTTCACAAATAGTAACCA	AAAGGGAGGGTAAGTGTGGA	36.4	50.0	55.0	57.9	814 bp	1.0	40.1