Hotspot

psbA

LSC · 609-1670 · 1062 bp

Back to Tieghemella

Pi 0.000942

MarkerSeek score 55.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GAGCAATAACGCCCTCTTGA	AAATTCGTGTGCTTGGGAGT	50.0	45.0	58.0	58.0	1197 bp	1.0	79.7
psbA_p2	AAAGGAGCAATAACGCCCTC	AAATTCGTGTGCTTGGGAGT	50.0	45.0	58.2	58.0	1201 bp	1.0	77.9
psbA_p3	GAGCAATAACGCCCTCTTGA	AATTCGTGTGCTTGGGAGTC	50.0	50.0	58.0	58.5	1196 bp	1.0	76.1
psbA_p4	AAAGGAGCAATAACGCCCTC	AATTCGTGTGCTTGGGAGTC	50.0	50.0	58.2	58.5	1200 bp	1.0	74.3
psbA_p5	AGCAATAACGCCCTCTTGAT	AAATTCGTGTGCTTGGGAGT	45.0	45.0	57.3	58.0	1196 bp	1.0	74.2