Hotspot

petA

LSC · 66164-67126 · 963 bp

Back to Tieghemella

Pi 0

MarkerSeek score 40.00

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGCAGAATCGTGGATAGGGA	TGATGTTGATAAATCCGCGAGT	50.0	40.9	57.9	58.2	1063 bp	1.0	61.6
petA_p2	CATCCCGGGGATTCATCCTA	TGATGTTGATAAATCCGCGAGT	55.0	40.9	58.3	58.2	1103 bp	1.0	59.6
petA_p3	AGCAGAATCGTGGATAGGGA	TGATGTTGATAAATCCGCGAG	50.0	42.9	57.9	56.5	1063 bp	1.0	59.4
petA_p4	AGCAGAATCGTGGATAGGGA	GATGTTGATAAATCCGCGAGT	50.0	42.9	57.9	56.5	1062 bp	1.0	59.3
petA_p5	AGCAGAATCGTGGATAGGGA	ACTTGATGTTGATAAATCCGCG	50.0	40.9	57.9	57.5	1066 bp	1.0	59.0