Hotspot

psbE-petL

LSC · 68995-70286 · 1292 bp

Back to Tieghemella

Pi 0.000774

MarkerSeek score 59.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 1
pi: 0.000774
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1484-1485 bp	1.0	72.1
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1407-1408 bp	1.0	71.3
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1493-1494 bp	1.0	71.2
psbE-petL_p4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1498-1499 bp	1.0	71.1
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1492-1493 bp	1.0	71.0