Hotspot

ycf3-trnS-GGA

LSC · 48032-48896 · 865 bp

Back to Tieghemella

Pi 0.00116
MarkerSeek score 59.40
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
865
variable_sites
1
pi
0.00116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 975 bp 1.0 70.5
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 925 bp 1.0 70.2
ycf3-trnS-GGA_p3 ATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.6 58.2 974 bp 1.0 67.5
ycf3-trnS-GGA_p4 TTTATCCGCGATCTAGGCAT ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.6 58.2 973 bp 1.0 67.5
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTG 47.6 50.0 58.0 58.5 927 bp 1.0 67.4