Hotspot

psaA-ycf3

LSC · 45307-46055 · 749 bp

Back to Tieghemella

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	927 bp	1.0	78.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	909 bp	1.0	78.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	942 bp	1.0	78.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	929 bp	1.0	78.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	923 bp	1.0	77.1