Hotspot

trnE-UUC-trnT-GGU

LSC · 33815-34683 · 869 bp

Back to Tieghemella

Pi 0

MarkerSeek score 58.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AAGAGAGGTGTCCTGAACCA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	958-959 bp	1.0	79.2
trnE-UUC-trnT-GGU_p2	GCCTCCTTGAAAGAGAGGTG	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	968-969 bp	1.0	79.2
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1009-1010 bp	1.0	78.8
trnE-UUC-trnT-GGU_p4	AAGAGAGGTGTCCTGAACCA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	937-938 bp	1.0	78.7
trnE-UUC-trnT-GGU_p5	GCCTCCTTGAAAGAGAGGTG	CCATGGCGTTACTCTACCAC	55.0	55.0	57.9	58.1	947-948 bp	1.0	78.7