Hotspot

trnK-UUU-rps16

LSC · 4501-5339 · 839 bp

Back to Tieghemella

Pi 0
MarkerSeek score 59.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
839
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAGGCGGAGGTTTTTAAGGA 50.0 45.0 58.2 57.0 930-976 bp 1.0 71.1
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AGGCGGAGGTTTTTAAGGAA 50.0 45.0 58.2 57.0 929-975 bp 1.0 71.1
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAGGCGGAGGTTTTTAAGGA 50.0 45.0 58.2 57.0 929-975 bp 1.0 71.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGGCGGAGGTTTTTAAGGAA 50.0 45.0 58.2 57.0 928-974 bp 1.0 71.1
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC AGGCGGAGGTTTTTAAGGAA 50.0 45.0 57.8 57.0 1014-1060 bp 1.0 70.6