Hotspot

matK-trnK-UUU

LSC · 3728-4463 · 736 bp

Back to Tieghemella

Pi 0

MarkerSeek score 59.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACGAAGTTGTGTTGCTGAGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.7	58.2	811-816 bp	1.0	76.7
matK-trnK-UUU_p2	ACGAAGTTGTGTTGCTGAGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.7	58.2	810-815 bp	1.0	76.7
matK-trnK-UUU_p3	ACGAAGTTGTGTTGCTGAGA	GGGTTGCTAACTCAACGGTA	45.0	50.0	57.7	57.5	824-829 bp	1.0	74.3
matK-trnK-UUU_p4	ACGAAGTTGTGTTGCTGAGA	TTCGGGTTGCTAACTCAACG	45.0	50.0	57.7	58.5	827-832 bp	1.0	74.0
matK-trnK-UUU_p5	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.2	58.2	861-866 bp	1.0	72.9