primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	GAGCAATAACGCCCTCTTGA	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.042919	609	1670	1197-1197	1.000000	79.7
psbA_p2	psbA	2	AAAGGAGCAATAACGCCCTC	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.267306	609	1670	1201-1201	1.000000	77.9
psbA_p3	psbA	3	GAGCAATAACGCCCTCTTGA	AATTCGTGTGCTTGGGAGTC	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.500418	609	1670	1196-1196	1.000000	76.1
psbA_p4	psbA	4	AAAGGAGCAATAACGCCCTC	AATTCGTGTGCTTGGGAGTC	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.724805	609	1670	1200-1200	1.000000	74.3
psbA_p5	psbA	5	AGCAATAACGCCCTCTTGAT	AAATTCGTGTGCTTGGGAGT	20	20	45.0	45.0	57.3	58.0	0.0	0.0	0.736382	609	1670	1196-1196	1.000000	74.2
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACGAAGTTGTGTTGCTGAGA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	57.7	58.2	0.0	17.1	0.477948	3728	4463	811-816	1.000000	76.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACGAAGTTGTGTTGCTGAGA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.7	58.2	0.0	17.1	0.477948	3728	4463	810-815	1.000000	76.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACGAAGTTGTGTTGCTGAGA	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	57.7	57.5	0.0	0.0	0.770285	3728	4463	824-829	1.000000	74.3
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACGAAGTTGTGTTGCTGAGA	TTCGGGTTGCTAACTCAACG	20	20	45.0	50.0	57.7	58.5	0.0	0.0	0.805169	3728	4463	827-832	1.000000	74.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3728	4463	861-866	1.000000	72.9
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAGGCGGAGGTTTTTAAGGA	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4501	5339	930-976	1.000000	71.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	AGGCGGAGGTTTTTAAGGAA	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4501	5339	929-975	1.000000	71.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AAGGCGGAGGTTTTTAAGGA	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4501	5339	929-975	1.000000	71.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	AGGCGGAGGTTTTTAAGGAA	20	20	50.0	45.0	58.2	57.0	20.3	0.0	1.132019	4501	5339	928-974	1.000000	71.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	AGGCGGAGGTTTTTAAGGAA	20	20	50.0	45.0	57.8	57.0	0.0	0.0	1.197899	4501	5339	1014-1060	1.000000	70.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.081289	6475	8502	2108-2108	1.000000	79.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCCTGTCTTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.210236	6475	8502	2112-2112	1.000000	78.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6475	8502	2101-2101	1.000000	78.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCACAACGGATCCTGTCTTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	10.8	16.9	0.261105	6475	8502	2122-2122	1.000000	78.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GTCTTTCAAGTCGCACGTTG	AAATTTAAATGGGGCGTGGC	20	20	50.0	45.0	58.0	57.6	0.0	0.4	0.400118	6475	8502	2165-2165	1.000000	77.0
rps2_p1	rps2	1	TGAGGTTCACAAATAGTAACCA	GCCAATCCACGGTAGAAGAG	22	20	36.4	55.0	55.0	58.1	5.9	0.0	5.030078	17434	18144	900-901	1.000000	40.2
rps2_p2	rps2	2	TGAGGTTCACAAATAGTAACCAA	GCCAATCCACGGTAGAAGAG	23	20	34.8	55.0	55.8	58.1	5.9	0.0	5.273239	17434	18144	900-901	1.000000	40.2
rps2_p3	rps2	3	TTGAGGTTCACAAATAGTAACCA	GCCAATCCACGGTAGAAGAG	23	20	34.8	55.0	55.8	58.1	5.9	0.0	5.273239	17434	18144	901-902	1.000000	40.2
rps2_p4	rps2	4	GGTTCACAAATAGTAACCAATTCCA	GCCAATCCACGGTAGAAGAG	25	20	36.0	55.0	57.8	58.1	0.0	0.0	5.280675	17434	18144	897-898	1.000000	40.2
rps2_p5	rps2	5	TGAGGTTCACAAATAGTAACCA	AAAGGGAGGGTAAGTGTGGA	22	20	36.4	50.0	55.0	57.9	5.9	0.0	5.099054	17434	18144	814-814	1.000000	40.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AAGAGAGGTGTCCTGAACCA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.109124	33815	34683	958-959	1.000000	79.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GCCTCCTTGAAAGAGAGGTG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.9	58.0	19.9	0.0	0.112448	33815	34683	968-969	1.000000	79.2
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	33815	34683	1009-1010	1.000000	78.8
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AAGAGAGGTGTCCTGAACCA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.167221	33815	34683	937-938	1.000000	78.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCCTCCTTGAAAGAGAGGTG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.9	58.1	19.9	0.0	0.170545	33815	34683	947-948	1.000000	78.7
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	45307	46055	927-927	1.000000	78.8
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	45307	46055	909-909	1.000000	78.8
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.214484	45307	46055	942-942	1.000000	78.4
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	45307	46055	929-929	1.000000	78.0
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	45307	46055	923-923	1.000000	77.1
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	48032	48896	975-975	1.000000	70.5
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	58.0	57.8	8.2	5.6	1.233148	48032	48896	925-925	1.000000	70.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	ATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.6	58.2	8.2	0.0	1.571225	48032	48896	974-974	1.000000	67.5
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTATCCGCGATCTAGGCAT	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.6	58.2	8.2	0.0	1.571225	48032	48896	973-973	1.000000	67.5
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTG	21	20	47.6	50.0	58.0	58.5	8.2	0.0	1.587330	48032	48896	927-927	1.000000	67.4
petA_p1	petA	1	AGCAGAATCGTGGATAGGGA	TGATGTTGATAAATCCGCGAGT	20	22	50.0	40.9	57.9	58.2	0.0	8.2	2.295703	66164	67126	1063-1063	1.000000	61.6
petA_p2	petA	2	CATCCCGGGGATTCATCCTA	TGATGTTGATAAATCCGCGAGT	20	22	55.0	40.9	58.3	58.2	26.2	8.2	2.551012	66164	67126	1103-1103	1.000000	59.6
petA_p3	petA	3	AGCAGAATCGTGGATAGGGA	TGATGTTGATAAATCCGCGAG	20	21	50.0	42.9	57.9	56.5	0.0	8.2	2.579951	66164	67126	1063-1063	1.000000	59.4
petA_p4	petA	4	AGCAGAATCGTGGATAGGGA	GATGTTGATAAATCCGCGAGT	20	21	50.0	42.9	57.9	56.5	0.0	8.2	2.582174	66164	67126	1062-1062	1.000000	59.3
petA_p5	petA	5	AGCAGAATCGTGGATAGGGA	ACTTGATGTTGATAAATCCGCG	20	22	50.0	40.9	57.9	57.5	0.0	0.0	2.624468	66164	67126	1066-1066	1.000000	59.0
petA-psbJ_p1	petA-psbJ	1	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.8	57.6	0.0	4.1	0.586979	67127	68214	1198-1198	1.000000	77.0
petA-psbJ_p2	petA-psbJ	2	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.5	57.6	0.0	4.1	0.879177	67127	68214	1199-1199	1.000000	74.6
petA-psbJ_p3	petA-psbJ	3	GCATCTGTTGTTTTGGCACA	TGGTTCATATTCCGGATTGGG	20	21	45.0	47.6	57.8	57.8	0.0	4.1	1.444180	67127	68214	1206-1206	1.000000	70.1
petA-psbJ_p4	petA-psbJ	4	GCATCTGTTGTTTTGGCACA	GGTTCATATTCCGGATTGGGT	20	21	45.0	47.6	57.8	57.8	0.0	4.1	1.445681	67127	68214	1205-1205	1.000000	70.1
petA-psbJ_p5	petA-psbJ	5	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	56.8	57.6	5.1	4.1	1.527465	67127	68214	1148-1148	1.000000	69.5
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	68995	70286	1484-1485	1.000000	72.1
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	68995	70286	1407-1408	1.000000	71.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	68995	70286	1493-1494	1.000000	71.2
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	68995	70286	1498-1499	1.000000	71.1
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	68995	70286	1492-1493	1.000000	71.0
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	73416	74206	947-950	1.000000	75.0
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	73416	74206	946-949	1.000000	75.0
rpl20-rps12_p3	rpl20-rps12	3	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACTTGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.776040	73416	74206	910-913	1.000000	74.1
rpl20-rps12_p4	rpl20-rps12	4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACTTGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.776040	73416	74206	909-912	1.000000	74.1
rpl20-rps12_p5	rpl20-rps12	5	TCCCCGTCTAATTCTGGTCA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.781830	73416	74206	879-882	1.000000	74.0
trnI-GAU_p1	trnI-GAU	1	GATGGGGTTTCTCTCGCTTT	GAGCATGCATGAAGGAGTCA	20	20	50.0	50.0	57.9	58.0	0.0	30.6	0.149447	107191	108205	1123-1123	1.000000	78.9
trnI-GAU_p2	trnI-GAU	2	GATGGGGTTTCTCTCGCTTT	GCATGCATGAAGGAGTCAGA	20	20	50.0	50.0	57.9	58.0	0.0	30.6	0.149447	107191	108205	1121-1121	1.000000	78.9
trnI-GAU_p3	trnI-GAU	3	GATGGGGTTTCTCTCGCTTT	TGCATGAAGGAGTCAGATGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.149447	107191	108205	1118-1118	1.000000	78.9
trnI-GAU_p4	trnI-GAU	4	GATGGGGTTTCTCTCGCTTT	TCTATTCTTTTCCCTGGCGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.153730	107191	108205	1094-1094	1.000000	78.8
trnI-GAU_p5	trnI-GAU	5	GATGGGGTTTCTCTCGCTTT	AGCATGCATGAAGGAGTCAG	20	20	50.0	50.0	57.9	58.0	0.0	30.6	0.155161	107191	108205	1122-1122	1.000000	78.8
ndhF-rpl32_p1	ndhF-rpl32	1	AAAAGAAGAAGCCCCACTCC	AAAAGCTTTTAAGGCTGCCT	20	20	50.0	40.0	57.7	56.1	0.0	22.7	2.191124	117311	118376	1224-1248	1.000000	64.1
ndhF-rpl32_p2	ndhF-rpl32	2	AAAAGAAGAAGCCCCACTCC	AAAGCTTTTAAGGCTGCCTT	20	20	50.0	40.0	57.7	56.1	0.0	34.0	2.191124	117311	118376	1223-1247	1.000000	64.1
ndhF-rpl32_p3	ndhF-rpl32	3	CTGGAAGTGGAACAAACGGT	AAAAGCTTTTAAGGCTGCCT	20	20	50.0	40.0	58.3	56.1	0.0	22.7	2.221123	117311	118376	1190-1214	1.000000	63.9
ndhF-rpl32_p4	ndhF-rpl32	4	CTGGAAGTGGAACAAACGGT	AAAGCTTTTAAGGCTGCCTT	20	20	50.0	40.0	58.3	56.1	0.0	34.0	2.221123	117311	118376	1189-1213	1.000000	63.9
ndhF-rpl32_p5	ndhF-rpl32	5	AAACGGTATGATCCACGCAT	AAAGCTTTTAAGGCTGCCTT	20	20	45.0	40.0	57.7	56.1	0.0	34.0	2.222258	117311	118376	1176-1200	1.000000	63.9
rps15-ycf1_p1	rps15-ycf1	1	TCAACAGACCCTCGGTTTTC	TGGCAGTCGTTTCAGTATGT	20	20	50.0	45.0	57.8	57.2	0.0	0.0	1.081529	128702	129098	511-511	1.000000	71.8
rps15-ycf1_p2	rps15-ycf1	2	CAACAGACCCTCGGTTTTCT	TGGCAGTCGTTTCAGTATGT	20	20	50.0	45.0	57.7	57.2	0.0	0.0	1.087802	128702	129098	510-510	1.000000	71.8
rps15-ycf1_p3	rps15-ycf1	3	TCAACAGACCCTCGGTTTTC	TGGCAGTCGTTTCAGTATGTT	20	21	50.0	42.9	57.8	57.9	0.0	0.0	1.370164	128702	129098	511-511	1.000000	69.5
rps15-ycf1_p4	rps15-ycf1	4	CAACAGACCCTCGGTTTTCT	TGGCAGTCGTTTCAGTATGTT	20	21	50.0	42.9	57.7	57.9	0.0	0.0	1.376438	128702	129098	510-510	1.000000	69.5
rps15-ycf1_p5	rps15-ycf1	5	ACAGACCCTCGGTTTTCTTC	TGGCAGTCGTTTCAGTATGT	20	20	50.0	45.0	57.5	57.2	0.0	0.0	1.377222	128702	129098	508-508	1.000000	69.5
trnI-GAU_p1	trnI-GAU	1	GAGCATGCATGAAGGAGTCA	GATGGGGTTTCTCTCGCTTT	20	20	50.0	50.0	58.0	57.9	30.6	0.0	0.149447	140544	141558	1123-1123	1.000000	78.9
trnI-GAU_p2	trnI-GAU	2	GCATGCATGAAGGAGTCAGA	GATGGGGTTTCTCTCGCTTT	20	20	50.0	50.0	58.0	57.9	30.6	0.0	0.149447	140544	141558	1121-1121	1.000000	78.9
trnI-GAU_p3	trnI-GAU	3	TGCATGAAGGAGTCAGATGC	GATGGGGTTTCTCTCGCTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.149447	140544	141558	1118-1118	1.000000	78.9
trnI-GAU_p4	trnI-GAU	4	TCTATTCTTTTCCCTGGCGC	GATGGGGTTTCTCTCGCTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.153730	140544	141558	1094-1094	1.000000	78.8
trnI-GAU_p5	trnI-GAU	5	AGCATGCATGAAGGAGTCAG	GATGGGGTTTCTCTCGCTTT	20	20	50.0	50.0	58.0	57.9	30.6	0.0	0.155161	140544	141558	1122-1122	1.000000	78.8