Hotspot

ndhI-ndhG

SSC · 118938-119084 · 147 bp

Back to Tibetiodes

Pi 0.0186

MarkerSeek score 66.80

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 147
variable_sites: 4
pi: 0.0186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TCAAATTGCTTTGGGTCGGT	TACTACTCCCAGACCCCCTA	45.0	55.0	58.3	57.7	312-316 bp	1.0	77.2
ndhI-ndhG_p2	TCAAATTGCTTTGGGTCGGT	TCCCAACGAAAAGGCAGAAT	45.0	45.0	58.3	57.7	351-355 bp	1.0	77.1
ndhI-ndhG_p3	TCAAATTGCTTTGGGTCGGT	ATACTACTCCCAGACCCCCT	45.0	55.0	58.3	58.4	313-317 bp	1.0	76.7
ndhI-ndhG_p4	TCAAATTGCTTTGGGTCGGT	AATACTACTCCCAGACCCCC	45.0	55.0	58.3	57.5	314-318 bp	1.0	75.7
ndhI-ndhG_p5	CAAATTGCTTTGGGTCGGTT	TCCCAACGAAAAGGCAGAAT	45.0	45.0	57.5	57.7	350-354 bp	1.0	75.3