Hotspot

petA-psbJ

LSC · 63253-63970 · 718 bp

Back to Tibetiodes

Pi 0.0132

MarkerSeek score 68.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 14
pi: 0.0132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	CCGGATTGGGTTCATCTCTG	45.0	55.0	58.0	58.0	848-853 bp	1.0	81.7
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.9	58.0	838-843 bp	1.0	81.7
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	TCCGGATTGGGTTCATCTCT	45.0	50.0	58.0	58.1	849-854 bp	1.0	81.1
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.9	58.1	839-844 bp	1.0	81.1
petA-psbJ_p5	TCCATTACGTGTACAAGGCC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.6	58.0	854-859 bp	1.0	79.0