Hotspot

accD-psaI

LSC · 58527-59441 · 915 bp

Back to Tibetiodes

Pi 0.0189

MarkerSeek score 61.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 24
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGCGTTCTGAGTGAGT	GAGGGAAAATTAAGAGTTGTCA	45.0	36.4	57.4	54.0	999-1062 bp	1.0	45.9
accD-psaI_p2	AAAGGCGTTCTGAGTGAGTT	GAGGGAAAATTAAGAGTTGTCA	45.0	36.4	57.4	54.0	998-1061 bp	1.0	45.9
accD-psaI_p3	AAAAGGCGTTCTGAGTGAGT	AGGGAAAATTAAGAGTTGTCA	45.0	33.3	57.4	52.6	998-1061 bp	1.0	45.9
accD-psaI_p4	AAAGGCGTTCTGAGTGAGTT	AGGGAAAATTAAGAGTTGTCA	45.0	33.3	57.4	52.6	997-1060 bp	1.0	45.9
accD-psaI_p5	AAAGGCGTTCTGAGTGAGTT	GAGGGAAAATTAAGAGTTGTCAT	45.0	34.8	57.4	54.6	998-1061 bp	1.0	45.9