Hotspot

trnG-UCC-psbD

LSC · 30786-32479 · 1694 bp

Back to Tibetiodes

Pi 0.0335

MarkerSeek score 81.50

Primer available yes

Label trnG-UCC-psbD

Feature Metadata

label_name: trnG-UCC-psbD
feature_type: igs
region: LSC
length_bp: 1694
variable_sites: 77
pi: 0.0335
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbD_p1	AATGAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1855-2034 bp	1.0	79.2
trnG-UCC-psbD_p2	ATGAAACCGAAAGACCCCTT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1854-2033 bp	1.0	79.2
trnG-UCC-psbD_p3	AATGAAACCGAAAGACCCCT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.0	58.1	1873-2052 bp	1.0	78.3
trnG-UCC-psbD_p4	ATGAAACCGAAAGACCCCTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.0	58.1	1872-2051 bp	1.0	78.3
trnG-UCC-psbD_p5	GAATGAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	47.6	55.0	58.2	58.0	1856-2035 bp	1.0	77.3