Hotspot

trnE-UUC-rpoB

LSC · 11732-12746 · 1015 bp

Back to Tibetiodes

Pi 0.0173

MarkerSeek score 62.70

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 24
pi: 0.0173
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.0	57.6	1147-1164 bp	1.0	64.4
trnE-UUC-rpoB_p2	TAACCACTGGACCATAGGGG	TCAAACCCTGATCAATGAACCT	55.0	40.9	58.1	57.6	1106-1123 bp	1.0	63.8
trnE-UUC-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.1	57.6	1137-1154 bp	1.0	63.6
trnE-UUC-rpoB_p4	TTGAACCCTCGTTTTCTCCG	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.1	57.6	1142-1159 bp	1.0	63.6
trnE-UUC-rpoB_p5	GGGACTTGAACCCTCGTTTT	ACCCTGATCAATGAACCTACA	50.0	42.9	58.0	56.3	1143-1160 bp	1.0	62.2