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trnK-UUU-rps16

LSC · 4208-5018 · 811 bp

Back to Tibetiodes

Pi 0.00748

MarkerSeek score 63.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 9
pi: 0.00748
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	962-965 bp	1.0	76.9
trnK-UUU-rps16_p2	CCGCACTTAAAAGCCGAGTA	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	956-959 bp	1.0	76.7
trnK-UUU-rps16_p3	TCGCGGTCTTCCAAACTTTA	TTCCTTGAAAAAGGCGCTCA	45.0	45.0	57.5	58.3	1028-1031 bp	1.0	74.7
trnK-UUU-rps16_p4	CGCGGTCTTCCAAACTTTAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.4	58.3	1027-1030 bp	1.0	74.2
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	TCCTTGAAAAAGGCGCTCAG	50.0	50.0	58.3	58.8	961-964 bp	1.0	73.3