Hotspot

ycf1-rps15

Genome · 109860-110240 · 381 bp

Back to Tibetia

Pi 0.007

MarkerSeek score 51.60

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: Genome
length_bp: 381
variable_sites: 4
pi: 0.007
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ACCCATAATGGAAGCCGTTT	TGAAATTCAACGGATCCGGG	45.0	50.0	57.5	58.3	533-562 bp	1.0	77.0
ycf1-rps15_p2	ACCCATAATGGAAGCCGTTT	CCGGGGTTTTCTTCTTCCTT	45.0	50.0	57.5	57.7	518-547 bp	1.0	76.8
ycf1-rps15_p3	ACCCATAATGGAAGCCGTTT	ATCCGGGGTTTTCTTCTTCC	45.0	50.0	57.5	57.5	520-549 bp	1.0	75.0
ycf1-rps15_p4	ACCCATAATGGAAGCCGTTT	GGATCCGGGGTTTTCTTCTT	45.0	50.0	57.5	57.5	522-551 bp	1.0	75.0
ycf1-rps15_p5	ACCCATAATGGAAGCCGTTT	TCCGGGGTTTTCTTCTTCCT	45.0	50.0	57.5	58.6	519-548 bp	1.0	74.7