Hotspot

rpl23-trnI-CAU

Genome · 83002-83933 · 932 bp

Back to Tibetia

Pi 0.0265
MarkerSeek score 62.00
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
Genome
length_bp
932
variable_sites
37
pi
0.0265
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 CCTAGTTGATCCTGATTCGACA ATTGGCGAAGTCGTAGGTTC 45.5 50.0 57.3 58.0 879-1065 bp 1.0 65.7
rpl23-trnI-CAU_p2 TGTCCTAGTTGATCCTGATTCG ATTGGCGAAGTCGTAGGTTC 45.5 50.0 57.3 58.0 882-1068 bp 1.0 65.6
rpl23-trnI-CAU_p3 CCTAGTTGATCCTGATTCGACA CATCCATGGCTGAACGGTTA 45.5 50.0 57.3 58.0 915-1101 bp 1.0 65.3
rpl23-trnI-CAU_p4 TGTCCTAGTTGATCCTGATTCG CATCCATGGCTGAACGGTTA 45.5 50.0 57.3 58.0 918-1104 bp 1.0 65.3
rpl23-trnI-CAU_p5 CCTAGTTGATCCTGATTCGACA GTGTAAGCATCCATGGCTGA 45.5 50.0 57.3 58.0 922-1108 bp 1.0 65.2