Hotspot

rps19-rpl2

Genome · 79917-81160 · 1244 bp

Back to Tibetia

Pi 0.0241

MarkerSeek score 52.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: Genome
length_bp: 1244
variable_sites: 45
pi: 0.0241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CCGGAAGTTTCGAGTAGGTG	AGATGAAGGAACGCGAAGAC	55.0	50.0	58.0	58.0	2154-2168 bp	1.0	85.8
rps19-rpl2_p2	CCGGAAGTTTCGAGTAGGTG	CAGATGAAGGAACGCGAAGA	55.0	50.0	58.0	58.0	2155-2169 bp	1.0	85.8
rps19-rpl2_p3	CCGGAAGTTTCGAGTAGGTG	TAAGCCAGATGAAGGAACGC	55.0	50.0	58.0	58.0	2160-2174 bp	1.0	85.7
rps19-rpl2_p4	CCGGAAGTTTCGAGTAGGTG	ACGCGAAGACCTAGGATGTA	55.0	50.0	58.0	58.0	2144-2158 bp	1.0	85.5
rps19-rpl2_p5	TGGCCTACCATACGATCTGT	AGATGAAGGAACGCGAAGAC	50.0	50.0	57.9	58.0	2117-2131 bp	1.0	85.2