Hotspot

trnQ-UUG-accD

Genome · 54027-54507 · 481 bp

Back to Tibetia

Pi 0.0166

MarkerSeek score 54.40

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: Genome
length_bp: 481
variable_sites: 12
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	AGCATTGAGTTAAACCACCGT	55.0	42.9	58.1	58.1	534-549 bp	1.0	72.0
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	GCATTGAGTTAAACCACCGT	55.0	45.0	58.1	56.6	533-548 bp	1.0	70.3
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	GAGCATTGAGTTAAACCACCG	55.0	47.6	58.1	57.5	535-550 bp	1.0	69.4
trnQ-UUG-accD_p4	GAGGTTCGAATCCTTCCGTC	GCATTGAGTTAAACCACCGTT	55.0	42.9	58.1	57.4	533-548 bp	1.0	68.1
trnQ-UUG-accD_p5	GAGGTTCGAATCCTTCCGTC	AGCATTGAGTTAAACCACCG	55.0	45.0	58.1	56.3	534-549 bp	1.0	67.9