Hotspot

atpH-atpF

Genome · 47033-47604 · 572 bp

Back to Tibetia

Pi 0.0035

MarkerSeek score 50.50

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 572
variable_sites: 3
pi: 0.0035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	AGCAGAGGACAAAATACGGG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	734-736 bp	1.0	77.6
atpH-atpF_p2	GCAGAGGACAAAATACGGGG	GATGGCCAGTAACTCAAGCA	55.0	50.0	58.6	57.9	733-735 bp	1.0	75.9
atpH-atpF_p3	AGCAGAGGACAAAATACGGG	CGGATGGCCAGTAACTCAAG	50.0	55.0	57.6	58.3	736-738 bp	1.0	75.8
atpH-atpF_p4	AGCAGAGGACAAAATACGGG	TGGCCAGTAACTCAAGCAAA	50.0	45.0	57.6	57.6	732-734 bp	1.0	75.7
atpH-atpF_p5	AGCAGAGGACAAAATACGGG	ATCAAACCCGAAACTTCCGG	50.0	50.0	57.6	58.5	756-758 bp	1.0	74.7