Hotspot

ycf1

SSC · 125411-131023 · 5613 bp

Back to Tiarella

Pi 0.0043

MarkerSeek score 42.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 36
pi: 0.0043
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGTGTATTGATCCGAGGA	CCTTTTGGTTCTCCCCGAAA	42.9	50.0	56.5	58.0	629-653 bp	1.0	63.5
ycf1_p2	AGTTGTGTATTGATCCGAGGA	AGTTTGGGGAATGGAAGCTG	42.9	50.0	56.5	58.1	654-678 bp	1.0	62.9
ycf1_p3	AGTTGTGTATTGATCCGAGGA	GTCTGAAACCTTGGCACAGA	42.9	50.0	56.5	58.0	745-769 bp	1.0	62.8
ycf1_p4	AGTTGTGTATTGATCCGAGGA	CAGTTTGGGGAATGGAAGCT	42.9	50.0	56.5	58.1	655-679 bp	1.0	62.8
ycf1_p5	AGTTGTGTATTGATCCGAGGA	GCGCTCAAAGACGTAACCTA	42.9	50.0	56.5	58.0	1315 bp	0.333333	32.9