Hotspot

ndhF

SSC · 111686-113923 · 2238 bp

Back to Tiarella

Pi 0.00536

MarkerSeek score 46.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 17
pi: 0.00536
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTCTGGTGACCCGTCTTTTC	ACGGATATCATAGCGTCTCCT	55.0	47.6	58.2	57.9	2921-2938 bp	1.0	70.6
ndhF_p2	TGAAAAACCTCTGGTGACCC	ACGGATATCATAGCGTCTCCT	50.0	47.6	57.6	57.9	2929-2946 bp	1.0	69.4
ndhF_p3	CTCTGGTGACCCGTCTTTTC	GACGGATATCATAGCGTCTCC	55.0	52.4	58.2	57.6	2922-2939 bp	1.0	68.0
ndhF_p4	TGAAAAACCTCTGGTGACCC	GACGGATATCATAGCGTCTCC	50.0	52.4	57.6	57.6	2930-2947 bp	1.0	66.7
ndhF_p5	GTGACCCGTCTTTTCGACTA	ACGGATATCATAGCGTCTCCT	50.0	47.6	57.3	57.9	2916-2933 bp	1.0	66.6