Hotspot

ccsA-psaC

SSC · 116822-118682 · 1861 bp

Back to Tiarella

Pi 0.00485

MarkerSeek score 57.50

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 1861
variable_sites: 13
pi: 0.00485
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	TGTGGCTTCTATGGGGTTTC	CAGAAGACTGTGTCGGTTGT	50.0	50.0	57.5	57.8	2068-2071 bp	1.0	74.8
ccsA-psaC_p2	TGTGGCTTCTATGGGGTTTC	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.5	57.7	2048-2051 bp	1.0	74.0
ccsA-psaC_p3	TGTGGCTTCTATGGGGTTTC	GAGATGTGAATCCGCCTGTC	50.0	55.0	57.5	58.4	2046-2049 bp	1.0	73.2
ccsA-psaC_p4	TGTGGCTTCTATGGGGTTTC	GCCTGTCCAACGGATTTCTT	50.0	50.0	57.5	58.5	2033-2036 bp	1.0	72.9
ccsA-psaC_p5	GTGGCTTCTATGGGGTTTCT	CAGAAGACTGTGTCGGTTGT	50.0	50.0	57.2	57.8	2067-2070 bp	1.0	72.4