Hotspot

ndhF-rpl32

SSC · 113924-115037 · 1114 bp

Back to Tiarella

Pi 0.00748

MarkerSeek score 65.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1114
variable_sites: 12
pi: 0.00748
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCCGTTGGAAAAAGGAGAA	TTAACGCTGCCCAATATCCC	45.0	50.0	57.9	58.0	1288-1298 bp	1.0	81.4
ndhF-rpl32_p2	TTGCCGTTGGAAAAAGGAGA	TTAACGCTGCCCAATATCCC	45.0	50.0	57.9	58.0	1289-1299 bp	1.0	81.4
ndhF-rpl32_p3	AGACAGGAACAGGAAGTGGA	TTAACGCTGCCCAATATCCC	50.0	50.0	57.9	58.0	1253-1263 bp	1.0	81.1
ndhF-rpl32_p4	GTTGCCGTTGGAAAAAGGAG	TTAACGCTGCCCAATATCCC	50.0	50.0	57.9	58.0	1290-1300 bp	1.0	80.8
ndhF-rpl32_p5	TGCCGTTGGAAAAAGGAGAA	AAAAGCTTTTAACGCTGCCC	45.0	45.0	57.9	57.9	1296-1306 bp	1.0	80.4